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KMID : 1145120190170030353
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Asian Journal of Beauty and Cosmetology
2019 Volume.17 No. 3 p.353 ~ p.363
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Effect of the Pine Cone Extract Phytochemical and Physiological activity on HaCaT Cells
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Kim Jin
Park Jing-Gi
Lee Chang-Moon
Kim Su-Gwan
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Abstract
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Purpose: This study examines the properties and potential commercial value of the pine cones extract when used as bioactive and cosmetics ingredients.
Method: Pine cones were extracted with hot water (WE), 70% ethanol (EE), and supercritical fluid extraction (SFE) to examine their antioxidant properties on the basis of their total polyphenol content, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity, and gas chromatography-mass spectrometry (GC-MS) analysis. The antibacterial properties of the pine cone extract were also studied on 2 types of bacteria. In addition, this study examined the nitric oxide (NO) inhibition effect using RAW 264.7 cells, in which the inflammatory reaction was induced by lipopolysaccharides (LPS), and cell migration activity in the HeCaT cells.
Results: The extract obtained with SFE exhibited high antioxidative ability even at low concentration (0.125%). The antimicrobial activity of the of SFE pine cone extract was 12.1±1.04 mm for Staphylococcus aureus (S. aureus) and 11.8±0.45 mm for Staphylococcus epidermidis (S. epidermidis). Cytotoxicity evaluation showed cell viability above 80% in all extracts. During the antiinflammatory test, the nitric oxide expression level was low, and it was confirmed by the antiinflammatory effect. The results of the stability test showed that emulsions containing SFE extract were very stable. The pH and high temperature (40℃) of each emulsion containing SFE extract did not considerably change for 30 days.
Conclusion: These findings suggest that SFE extract is a possible cosmeceutical material with potent skin regeneration and antiinflammatory properties.
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KEYWORD
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Pine cones, Phytochemical, Supercritical fluid extraction, Anti?inflammatory, Cosmeceutical
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